Cultivation
Effect of Propagation Techniques on Growth, Development, Oil Yield, and Quality of Medicinal Cannabis (Cannabis sativa) Found in Lusikisiki, Eastern Cape, South Africa
Abstract This study investigated the influence of cutting techniques on the growth, development, yield, and oil quality of Cannabis sativa found in the Eastern Cape Province. The greenhouse pot experiment was conducted at Dohne [...]
The correlation of leaf parameters with flower yield and cannabinoid accumulation in medical cannabis under different LED light spectra
Abstract Light is a critical factor in cannabis cultivation, influencing photosynthesis, secondary metabolite production, and photomorphogenesis. Despite its significance, the metabolic interactions between leaves and flowers under varying light spectra remain inadequately understood. Here, [...]
LED interlighting: a cost-effective strategy to increase yield and chemical uniformity of Cannabis sativa at commercial scale
Abstract Cannabinoid uniformity plays an important role in the quality and marketability of cannabis products. Light is an important environmental factor determining increased yield and improved secondary compound production, while energy consumption is a [...]
Examining the “Night Break” Method in Cannabis sativa Horticulture: Vegetative Daily Light Integral Affects Yield of Extractable Biomass in C. sativa
Abstract Cannabis sativa is a short-day (SD) plant, producing inflorescences when the daily scotoperiod (period of darkness) exceeds approximately 10 h of length. As such, the vegetative photoperiod is typically maintained at 16 to [...]
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Single trichome phytocannabinomics of two different cannabis varieties
Abstract The present study investigates the phytocannabinoid profiles of Cannabis sativa L. by analyzing individual glandular trichomes from both a medicinal cannabis variety (FM2) and a high-CBD (cannabidiol) hemp variety. The analysis was performed [...]
Bracts, Buds, and Biases: Uncovering Gaps in Trichome Density Quantification and Cannabinoid Concentration in Cannabis sativa L.
Abstract Trichomes in cannabis (Cannabis sativa L.) are specialized structures responsible for cannabinoid and terpene biosynthesis, making their density a critical parameter for both research and industrial applications. However, consistent trichome density assessment remains [...]
Mother transformer: A High-Throughput, Cost-Effective in Planta Hairy Root Transformation Method for Cannabis
Abstract Background Hairy root (HR) transformation assays mediated by Agrobacterium rhizogenes, both in vitro and ex vitro, are essential tools in plant biotechnology and functional genomics. These assays can be significantly influenced by various [...]
Unravelling herbicide stress and its impact on metabolite profiling in Cannabis sativa: an investigative study
Abstract Background Cannabis sativa L., renowned for its versatility in pharmaceutical, textile, and cosmetic industries, is highly susceptible to several agronomic and environmental factors, particularly herbicides. These chemical agents, while commonly used for weed [...]