Cultivation
Examining the “Night Break” Method in Cannabis sativa Horticulture: Vegetative Daily Light Integral Affects Yield of Extractable Biomass in C. sativa
Abstract Cannabis sativa is a short-day (SD) plant, producing inflorescences when the daily scotoperiod (period of darkness) exceeds approximately 10 h of length. As such, the vegetative photoperiod is typically maintained at 16 to [...]
Soilless Growing Media for Cannabis Cultivation
Abstract Standardized pharmaceutical-grade cultivation of Cannabis sativa L. increasingly relies on soilless systems for precision, reproducibility, and regulatory compliance. This review examines the role of inert and semi-inert growing media in indoor cannabis production, [...]
Anthocyanin accumulation, inflorescence dry weight and total cannabidiol content have different temperature optima in Cannabis sativa
Abstract Background Limited information exists on how temperature affects phytocannabinoids and anthocyanin accumulation and inflorescence dry weight yield in Cannabis sativa. Understanding how temperature influences these traits is essential for refining cultivation practices, meeting [...]
Optimizing Growth Regulator Concentrations for Cannabis sativa L. Micropropagation
Abstract In this study, the effect of growth regulators on shoot proliferation and rooting were evaluated to develop an efficient micropropagation protocol for the Cannabis sativa L. cultivars ‘Cherry Soda’ and ‘Purple’. Apical meristems [...]
News
Bracts, Buds, and Biases: Uncovering Gaps in Trichome Density Quantification and Cannabinoid Concentration in Cannabis sativa L.
Abstract Trichomes in cannabis (Cannabis sativa L.) are specialized structures responsible for cannabinoid and terpene biosynthesis, making their density a critical parameter for both research and industrial applications. However, consistent trichome density assessment remains [...]
Mother transformer: A High-Throughput, Cost-Effective in Planta Hairy Root Transformation Method for Cannabis
Abstract Background Hairy root (HR) transformation assays mediated by Agrobacterium rhizogenes, both in vitro and ex vitro, are essential tools in plant biotechnology and functional genomics. These assays can be significantly influenced by various [...]
Unravelling herbicide stress and its impact on metabolite profiling in Cannabis sativa: an investigative study
Abstract Background Cannabis sativa L., renowned for its versatility in pharmaceutical, textile, and cosmetic industries, is highly susceptible to several agronomic and environmental factors, particularly herbicides. These chemical agents, while commonly used for weed [...]
Variability of total THC in greenhouse cultivated dried Cannabis
Abstract Natural variation in secondary metabolites is commonplace in organic matrices, including consumable fruits and vegetables. However, absolute concentration and variation of secondary metabolites are generally not of concern to consumers. Cannabis sativa and C. [...]